Title: Binding of antagonists of H| and H2 histaminę receptors to peripheral blood lymphocytes in patients suffering from duodenal ulcer and ulcerative colitis
- Artur Salomon, Leszek Paradowski, Maria Jasińska
- Original articles
- Polish Gastroenterology
- Start page:
- Final page:
- histamin, H1 and H2 receptors, blood lymphocytes, duodenal ulcer, ulcerative colitis
Material and methods: The binding of H1 receptor antagonist-clemastine and H2 receptor antagonist-ranitidine by lymphocytes of peripheral blood was invastigated in 3 groups of patients: 1) patients with duodenal ulcer disease n=20; 2) patients with ulcerative colitis n=20; 3) healthy volunteers n=20. The lymphocytes were isolated by centrifugation, concentrated in 2 ml 015 M. NaCl. Then after placing the solutions in specially prepared dialysis tubes (produced by Union Carbide) we dialysed them in 100 ml (ccm) of solution contai-ning 2.5x10-5 M. of clemastine or ranitidine. In all of the prepared solutions we noted the extinction befo-re and after incubation. Also using standard curves the concentration of clemastine and ranitidine in dialy-sate before and after incubation was denoted.
Results: The results were expressed in umol of clemastine or ranitidine bound by 2x106 lymphocytes. The role of H1 and H2 receptors in diseases of gastrointesinal tract is still not clear. The investigation shows that lymphocytes bind histaminę receptors in different quantities. In patients with duodenal ulcer lymphocytes bind morę H2 receptor antagonist-ranitidine, but in patients with ulcerative colitis they bind morę clemastinum - the H1 receptor antagonist.
Conclusions: Those differences in binding histaminę receptor antagonists may be a result of a different densi-tyof H1 and H2 receptors, which islikelyto besignificanttothe pathophysiology of both diseases.