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Articles

Title: Modern methods for diagnosis of dermatophyte infections in routine laboratory praxis

Author:
Anna Brillowska-Dąbrowska
Type:
Review articles
Language:
PL
Journal:
Medical Mycology
Year:
2009
Volume:
16
Number:
1
Start page:
47
Final page:
49
ISSN:
1232-986X
Keywords:
dermatophytes, Molecular DiagnosticTechniques
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Fungi belonging to the genera Microsporum, Trichophyton and Epidermophyton are the main causative agent of superficial mycosis.The micro- and macroscopic obser- vation in classical species identification requires pure culture, what due to the slow growth rate of dermatophytes causes 2-3 weeks waiting timefor the diagnosis. Molecular diagnostic methods, such as PCR, amplify DNA and don’t require the culture of pathogens, which make them especially useful for their application in routine laboratory praxis.The application of majority of commercially available DNA extraction methods is not possible, due to the structure of dermatophyte cell wall and examined specimens. Moreover, the proposed molecular procedures described in scientific publications usually are not relevant to the clinical needs. However, introduction of the diagnostic procedures elaborated by research groups would enable rational therapy of dermatophytosis. It is worth to notice, that elaborating of the molecular methods aims in increasing prevalence of laboratory diagnosis of dermatophytosis, but not eliminating of the classical methods.