Title: MSSCP technology as a tool for the detection of genetic differences in ERG11 gene in Candida albicans sensitive and resistant to azoles
- Anna Ślemp-Migiel, Joanna Katarzyna Strzelczyk, Magdalena Rother, Andrzej Wiczkowski
- Original articles
- Medical Mycology
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- Final page:
- Candida albicans, ERG1 1, MSSCP
Background: One of the mechanisms for resistance to azole antifungal drugs used in C. albicans therapy is the contribution of overexpression and point mutations in
ERG11 gene that codes 14a-demethylase (14DM) which is the primary target for the azole class of antifungals.These mutations can cause amino acids substitutions in the enzyme and alteration of 14DM sequence may lead to a decreased affinity of the enzyme for azoles.
Aim:The purpose of this study was to detect genetic differences present in collection of C. albicans isolatesdemonstrating resistance and sensitivity to azoles. Material and methods: C. albicans strains used in this study were isolated from clinical samples obtained from hospitalized patients.To identify such alterations ERG11 gene wasinvestigated by MSSCP technique (Multitemperature Single Strand Conformation Polymorphism). Results and conclusions: MSSCP analysis revealed the presence of genetic differences in ERG11 gene in analysed C. albicans strains.